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pcr select cdna subtraction kit  (TaKaRa)


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    TaKaRa pcr select cdna subtraction kit
    Pcr Select Cdna Subtraction Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 3399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clontech+pcr/Clontech+PCR-Select+cDNA+Subtraction+Kit/pm41987424-55-8-12
    Average 95 stars, based on 3399 article reviews
    pcr select cdna subtraction kit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Common and specific genomic sequences of avian and human extraintestinal pathogenic Escherichia coli as determined by genomic subtractive hybridization
    Article Snippet: .. The Clontech PCR-Select Bacterial Genome Subtraction Kit (ClontechLaboratories, Inc., Palo Alto, CA) was used for the SSHs according to the manufacturer's instructions. ..

    Article Title: A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa
    Article Snippet: .. The Clontech PCR-Select Bacterial Genome Subtraction kit was used to generate the SSH library of Rsa I fragments according to manufacturer's instructions but with a hybridization temperature of 73°C. .. The library of SSH PCR amplicons was cloned in pGEM-T Easy (Promega).

    Article Title: An unbiased approach to identify genes involved in development in a turtle with temperature-dependent sex determination
    Article Snippet: Testers and drivers were made from pooled RNA from stages 17–20 (two individuals per stage) from each experimental group from 2004 (stated above). cDNA synthesis was performed with the BD SMARTTM PCR cDNA Synthesis Kit (Clontech, Mountain View, CA) according to the manufacturer’s protocol. .. Three subtraction libraries were constructed with the Clontech PCR–Select TM cDNA Subtraction Kit (Clontech, Mountain View, CA) according to the manufacturer’s protocol except a PEG (PolyEthylene Glycol) precipitation followed by an ethanol wash was used to purify the PCR products after cDNA synthesis instead of the column chromatography. .. The resulting cDNA was ligated into a pGEM-T Easy vector and transformed into E. cloni ® 10 G electrocompetent cells (Lucigen) by the manufacturer’s protocol.

    Article Title: Efficient Anchoring of Erianthus arundinaceus Chromatin Introgressed into Sugarcane by Specific Molecular Markers
    Article Snippet: .. SSH was carried out as described in instructions for the Clontech PCR-Select Bacterial Genome Subtraction Kit (Clontech, Mountain View, CA, USA). ..

    other:

    Article Title: Analysis of the dermatophyte Trichophyton rubrum expressed sequence tags
    Article Snippet: The subtracted library was constructed following the protocols of the Clontech PCR-Selected cDNA Subtraction Kit.

    Article Title: Cloning, analysis and functional annotation of expressed sequence tags from the Earthworm Eisenia fetida
    Article Snippet: A Clontech PCR-Select TM cDNA subtraction kit (BD Biosciences) was then used to enrich for differentially expressed genes (Figure ).

    Article Title: Multifaceted natural drought response mechanisms in three elite date palm cultivars uncovered by expressed sequence tags analysis.
    Article Snippet: To identify genes differentially expressed during drought stress, cDNA libraries enriched for droughtresponsive transcripts were constructed for each date palm cultivar using the Clontech® PCR-SelectTM cDNA Subtraction Kit (Takara Bio Inc., California, USA).

    Article Title: Construction and analysis of cotton ( Gossypium arboreum L.) drought-related cDNA library
    Article Snippet: Such a screening was performed with the ClontechTM PCR-Select Differential Screening Kit (Clontech, USA).

    Hybridization:

    Article Title: A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa
    Article Snippet: .. The Clontech PCR-Select Bacterial Genome Subtraction kit was used to generate the SSH library of Rsa I fragments according to manufacturer's instructions but with a hybridization temperature of 73°C. .. The library of SSH PCR amplicons was cloned in pGEM-T Easy (Promega).

    Construct:

    Article Title: An unbiased approach to identify genes involved in development in a turtle with temperature-dependent sex determination
    Article Snippet: Testers and drivers were made from pooled RNA from stages 17–20 (two individuals per stage) from each experimental group from 2004 (stated above). cDNA synthesis was performed with the BD SMARTTM PCR cDNA Synthesis Kit (Clontech, Mountain View, CA) according to the manufacturer’s protocol. .. Three subtraction libraries were constructed with the Clontech PCR–Select TM cDNA Subtraction Kit (Clontech, Mountain View, CA) according to the manufacturer’s protocol except a PEG (PolyEthylene Glycol) precipitation followed by an ethanol wash was used to purify the PCR products after cDNA synthesis instead of the column chromatography. .. The resulting cDNA was ligated into a pGEM-T Easy vector and transformed into E. cloni ® 10 G electrocompetent cells (Lucigen) by the manufacturer’s protocol.

    cDNA Synthesis:

    Article Title: An unbiased approach to identify genes involved in development in a turtle with temperature-dependent sex determination
    Article Snippet: Testers and drivers were made from pooled RNA from stages 17–20 (two individuals per stage) from each experimental group from 2004 (stated above). cDNA synthesis was performed with the BD SMARTTM PCR cDNA Synthesis Kit (Clontech, Mountain View, CA) according to the manufacturer’s protocol. .. Three subtraction libraries were constructed with the Clontech PCR–Select TM cDNA Subtraction Kit (Clontech, Mountain View, CA) according to the manufacturer’s protocol except a PEG (PolyEthylene Glycol) precipitation followed by an ethanol wash was used to purify the PCR products after cDNA synthesis instead of the column chromatography. .. The resulting cDNA was ligated into a pGEM-T Easy vector and transformed into E. cloni ® 10 G electrocompetent cells (Lucigen) by the manufacturer’s protocol.

    Column Chromatography:

    Article Title: An unbiased approach to identify genes involved in development in a turtle with temperature-dependent sex determination
    Article Snippet: Testers and drivers were made from pooled RNA from stages 17–20 (two individuals per stage) from each experimental group from 2004 (stated above). cDNA synthesis was performed with the BD SMARTTM PCR cDNA Synthesis Kit (Clontech, Mountain View, CA) according to the manufacturer’s protocol. .. Three subtraction libraries were constructed with the Clontech PCR–Select TM cDNA Subtraction Kit (Clontech, Mountain View, CA) according to the manufacturer’s protocol except a PEG (PolyEthylene Glycol) precipitation followed by an ethanol wash was used to purify the PCR products after cDNA synthesis instead of the column chromatography. .. The resulting cDNA was ligated into a pGEM-T Easy vector and transformed into E. cloni ® 10 G electrocompetent cells (Lucigen) by the manufacturer’s protocol.



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